袁景和，副研究员。1999年获得四川大学光学专业硕士学位。2002年获得南开大学光学工程专业博士学位。2004年上海光机所国家强光光学重点实验室物理学博士后出站。现为中国科学院化学研究所分子纳米结构与纳米技术重点实验室副研究员。主要研究领域包括：生物医学光学成像,图像处理与模式识别。长期从事光学仪器研制，研制的主要仪器包括：超短激光脉冲激光激发的扫描共聚焦显微镜，并利用其实现超分辨STED、CARS和SERS显微成像；基于光学散射的激光微粒计数仪；基于手势和面部识别的人机交互系统；基于人眼识别的操作人员疲劳状态监测系统；基于光学微细加工的光学回归反射器等。现在主要研究领域为超分辨光学成像仪器和单分子数据处理。在显微光学成像、图像处理与模式识别等领域发表论文40余篇，获得国家发明专利6项。

代表性论文：

1.        Jinghe Yuan, Fanrong Xiao, Guiying Wang, Zhizhan Xu, “The imaging properties of coherent anti-Stokes Raman scattering (CARS) microscope”, Chinese physics, Vol.14, No.5, 1009 (2005).

2.        Jinghe Yuan, Ziqiang Hu, “High-order statistical blind deconvolution of spectroscopic data with Gauss-Newton algorithm”, Appl Spectrosc. 2006 Jun;60(6):692-7.

3.        Jinghe Yuan, Ziqiang Hu, Jingzuo Sun, “High-order cumulant based blind deconvolution of Raman spectra”, Appl Opt. 2005 Dec 10;44(35):7595-601.

4.        Jinghe Yuan，Zhizhan Xu，Sumei Li，Yanxin Zhang， “Novel approach for region merging and image segmentation for human-computer interaction”， Opt. Eng.，Vol. 42(8), 2277(2003).

5.        Jinghe Yuan, Shengjiang Chang, Yong Wang, Yanxin Zhang, "The Design and Fabrication of Micro-cube-corner array Retro-reflectors", Optics Communications, Vol.209, pp.75-83 (2002).

 

 

 

徐丽，副研究员。2006年7月于中科院动物所生殖生物学国家重点实验室获得生理学博士学位。 博士期间主要从事核酸疫苗的构建和生物学功能研究，在分子和细胞生物学方面有丰富的经验。 2006年博士毕业后进入中科院化学所分子纳米结构与纳米技术实验室，主要从事核酸适体的筛选和生物学功能研究，已经在复杂体系如细胞表面和单一体系如蛋白等多方面筛选出特异性核酸适体，并对其生物学功能进行了研究。 作为学术骨干参与核酸适体相关研究的项目两个，分别为卫生部重大专项项目“核酸适体在丙型病毒性肝炎的早期诊断和靶向治疗中的应用基础研究”和科学院知识创新工程重要方向项目“核酸适体分子工程新技术及其在疾病诊断治疗基础研究中的应用”。发表论文10余篇。

代表性论文：

1.        Shen QL, Xu L, Zhao LB, Wu DX, Fan YS, Zhou IL, Ouyang WH, Xu XC, Zhang Z, Lee T, Xiong B*, Hou S*, Tseng HR*, Fang XH*. Specific capture and release of circulating tumor cells using aptamer modified nanosubstrates. Advanced Materials, 2013, 25(16): 2368-2373.（并列第一作者）

2.        Liu QL, Xu L, Zhang XJ, Li N,  Zheng JP, Guan MR  Fang XH*, Wang CR* ; Shu CY*. Enhanced photodynamic efficiency of an aptamer-guided fullerene photosensitizer toward tumor cells, Chemisrty-An Asian Journal, 2013, in press, DOI: 10.1002/asia.201300039. （并列第一作者）

3.        Xu L, Zhang Z, Zhao ZL, Liu QL, Tan WH, Fang XH*. Cellular internalization and cytotoxicity of aptamers selected from lung cancer cell. American Journal of Biomedical Sciences, 2013, 5: 47-58.

4.        Wei YJ, Zhang XJ, Xu L, Yi SQ, Li Y, Fang XH, Liu HL*. The effect of cigarette smoke extract on thrombomodulin-thrombin binding: an atomic force microscopy study. SCINECE CHINA: Life Sciences, 2012, 55: 891-897.

5.        Liu QL, Guan MR, Xu L, Shu CY*, Zheng JP, Fang XH*, Yang YJ, and Wang CR*. The structural effect and mechanism study of C70-carboxyl-fullerenes as an efficient sensitizer against cancer cells. Small, 2012, 8: 2070-2077.

6.        张振，赵子龙，徐丽，吴新娇，朱海珍，谭蔚泓，方晓红*。基于核酸适体的丙肝病毒核心蛋白检测新方法。中国科学，2011, 41：1312-1318.

7.        Zhao ZL, Xu L, Shi XL, Tan WH, Fang XH*, Dihua SG. Recognition of subtype non-small cell lung cancer by DNA aptamer selected from living cells. Analyst, 2009, 134: 1808-1814.

8.        Shi XL, Xu L, Yu JP, Fang XH*. Study of inhibition effect of herceptin on interaction between heregulin and ErbB receptors HER3/HER2 by single-molecule force spectroscopy. Experimental cell research, 2009, 315: 2847-2855.

 

张振， 2004年毕业于武汉大学化学与分子科学学院；2009年获得北京大学化学与分子工程学院博士学位（直博）。目前研究工作包括：

（1） 发展基于核酸适体的疾病相关蛋白血清检测方法，主要是基于丙型病毒性肝炎血清学检测。

（2）利用纳米颗粒的催化效应发展高灵敏可视化检测方法

代表性论文：

1.        张振，赵子龙，徐丽，吴新娇，朱海珍，谭蔚泓，方晓红，“基于核酸适体的丙肝病毒核心蛋白检测新方法”，中国科学：化学，2011, 41, 1312.

2.        Zhen Zhang, Chunlai Chen, and Xin Sheng Zhao, “A simple and sensitive biosensor based on silver enhancement of aptamer-gold nanoparticle aggregation”, Electroanalysis, 2009, 21, 1316.

3.        Li Xu, Zhen Zhang, Zilong Zhao, Qiaoling Liu, Weihong Tan, Xiaohong Fang, “Cellular Internalization and Cytotoxicity of Aptamers Selected from Lung Cancer Cell”, Am. J. Biomed. Sci.2013, 5, 47.

4.        QlinglinShen, Li Xu, Libo Zhao, Dongxia Wu, Yunshan Fan, iliang Zhou, Wei-Han OuYang，XiaochunXu, Zhen Zhang, Tom Lee, Bin Xiong, ShuangHou, Hsian-Rong Tseng, Xiaohong Fang, “Specific capture and release of circulating tumor cells using aptamer modified nanosubstrates”, Adv. Mater., 2013,25, 2368.

5.        Jiajing Zhang, Zhen Zhang, XinNie, Zhenjiang Zhang, Xiaochun Wu,Chunying Chen, Xiaohong Fang, “A Label-Free Gold Nanocluster Fluorescent Probe for Protease Activity Monitoring”,J.Nanosci.Nanotechnol.2013, Accepted.

6.        MianqiXue, Zhen Zhang, Nan Zhu, Fangfang Wang, Xin Sheng Zhao, and Tingbing Cao，“Transfer printing of metal nanoparticles with controllable dimensions, placement and reproducible SERS effects”, Langmuir, 2009, 25, 4347.

7.        Zhaoqiang Wu, Shuling Gong, Cheng Li, Zhen Zhang, Weihua Huang,LingzhiMeng, Xiaoju Lu, Yongbing He, “Novel water-soluble fluorescent polymer containing recognition units: synthesis and interactions with PC12 cell”, European Polymer Journal, 2005, 41, 1985.

8.         Wei-Hua Huang, Wei Cheng, Zhen Zhang, Dai-Wen Pang, Zong-Li Wang, Jie-Ke Cheng, Da-Fu Cui, “Transport, location, and quantal release monitoring of single cells on a microfluidic devive”, Anal. Chem., 2004, 76, 483.

 

 

赵立波，分子纳米结构与纳米技术院重点实验室助理研究员（方晓红课题组）。2010年于武汉大学物理科学与技术学院获得博士学位。2010年至2013年在美国加州大学洛杉矶分校（UCLA）药理学系曾宪荣教授课题组任博士后，从事循环肿瘤细胞检测的研究。目前研究方向主要集中在核酸适配体的临床应用方面。

代表性论文：

1.        L. Zhao, Y.-T. Lu, Q. Shen, M.A. Garcia, D. Wu, S. Hou, M. Song, X. Xu, W.-H. OuYang, T. Lee, J. Huang, L.W.-K. Chung, M. Rettig, H.-R. Tseng, C. Shao, E.M. Posadas (2013) High Purity Prostate CTC Isolation Method for Whole Exome Sequencing, Adv.Mater., DOI: 10.1002/adma.201205237.

2.        Y.-T. Lu, L. Zhao, Q. Shen, M.A. Garcia, D. Wu, S. Hou, M. Song, X. Xu, W.-H. OuYang, T. Lee, X. Xuan, J. Huang, L.W.-K. Chung, M. Rettig, H.-R. Tseng, C. Shao, E.M. Posadas (2013) NanoVelcro Chip for CTC Enumeration in Prostate Cancer Patients, Methods, DOI：http://dx.doi.org/10.1016/j.ymeth.2013.06.019.

3.        Q. Shen, L. Xu, L. Zhao, D. Wu, Y. Fan, Y. Zhou, W.-H. OuYang, X. Xu, Z. Zhang, T. Lee, B. Xiong, S. Hou, H.-R. Tseng, X. Fang (2012) Specific Capture and Release of Circulating Tumor Cells Using Aptamer Modified Nanosubstrates, Adv. Mater., DOI: 10.1002/adma.201300082.

4.        S. Hou, L. Zhao, Q. Shen, J. Yu, C. Ng, X. Kong, D. Wu, M. Song, X. Shi, X. Xu, W.-H. OuYang, R. He, X.-Z. Zhao, B. Xiong, T. Lee, C. Brunicardi, M.A. Garcia, A. Ribas, R.S. Lo and H.-R. Tseng (2012) Polymer Nanofiber-Embedded Microchips for Detection, Isolation, and Molecular Analysis of Single Circulating Melanoma Cells, Angew. Chem. Int. Ed., 52, 3379-3383.

5.        S. Hou, H. Zhao, L. Zhao, Q. Shen, K.S. Wei, D. Y. Suh, A. Nakao, M.A. Garcia, M. Song, T. Lee, B. Xiong, S.-C. Luo, H.-R. Tseng, H.-H. Yu (2012) Capture and Stimulated Release of Circulating Tumor Cells on Polymer-Grafted Silicon Nanostructures, Adv. Mater., 25, 1547-1511.

6.        N. Zhang, Y. Deng, B. Cheng, L. Zhao, Q. Shen, R. He, L. Hong, W. Liu, S.S. Guo, K. Liu, H.-R. Tseng, B. Xiong, X.-Z. Zhao (2012) Electrospun TiO₂ Nanofiber-Based Cell Capture Assay for Detecting Circulating Tumor Cells from Colorectal and Gastric Cancer Patients, Adv. Mater.,24: 2756-2760.

7.        Z. Guo, M. Zhang, L. Zhao, S.S. Guo, X.-Z. Zhao (2011) Generation of Alginate Gel Particles with AuNPs Layers by Polydimethylsiloxan Template, Biomicrofluidics, 5: 026502.

8.        Y. Deng, N. Zhang, L. Zhao, X. Yu, X. Ji, W. Liu, S.S. Guo, K. Liu, X.-Z. Zhao (2011) Rapid Purification of Cell Encapsulated Hydrogel Beads from Oil Phase to Aqueous Phase in a Microfluidic Device, Lab Chip, 11: 4117-4121.

9.        S. Wang, K. Liu, J. Liu, Z.T.-F. Yu, X. Xu, L. Zhao, T. Lee, E.K. Lee, J. Reiss, Y.K. Lee, L.W.-K. Chung, J. Huang, M. Rettig, D. Seligson, K.N. Duraiswamy, C.K.-F. Shen, H.-R. Tseng (2011) Highly Efficient Capture of Circulating Tumor Cells by Using Nanostructured Silicon Substrate with Integrated Chaotic Micromixers, Angew. Chem. Int. Ed., 50: 3084-3088.